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[The qualitative analysis and quantitative analysis of purification of salvianolic acids by macroreticular resin]
1Key Laboratory of Pharmaceutics of Chinese Material Medica, Nan Fang Medical University, Guangzhou 510515, China.
Objective:
To purify salvianolic acids by macroreticular resin,then mensurate the contents of salvianolic acids and analyse the chromatogram with HPLC.
Method:
Make salvianolic acids with macroreticular resin; mensurate the content of Salvianolic acids with UV spestrophotometry: the control compound is protocaechuic aldehyde, and the wavelength is 281 nm. Analysis the chromatogram with HPLC, and compare the chromatogram in different technics: zorbax ODS column (4.6 mm x 250 mm, 5 microm), mobilephase: 1% aceticacid-water and methanol in different proportions, the wavelength is 281 nm.
Result:
The contents of salvianolic acids is 53.8%; HPLC chromatogram indicate that the method is reasonable to make salvianolic acids.
Conclusion:
Determination of contents and HPLC chromatogram can control the quality of Salvianolic acids more accurately.
