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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
Clonal derivation and characterization of human embryonic stem cell lines
Nico Heins1, Anders Lindahl, Ulrika Karlsson
1Cellartis AB, Arvid Wallgrens Backe 20, 413 46 Göteborg, Sweden.
Journal of Biotechnology
|December 6, 2005
Summary
We derived two new homogenous human embryonic stem cell (hESC) lines, SA002.5 and AS034.1.1, useful for drug development and studying trisomy 13.
Area of Science:
- Stem Cell Biology
- Genetics
- Developmental Biology
Background:
- Human embryonic stem cells (hESC) are typically heterogeneous.
- Subcloning can yield homogenous hESC populations.
- Previous hESC lines may contain karyotypic abnormalities.
Purpose of the Study:
- To derive and characterize novel homogenous hESC lines.
- To establish a diploid subclone from a trisomic hESC line.
- To provide tools for drug development and trisomy research.
Main Methods:
- Clonal derivation of hESC subclones.
- Karyotyping to assess chromosomal normality.
- Characterization of pluripotency markers (SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, Oct-4).
- Alkaline phosphatase activity and telomerase activity assays.
- In vitro and in vivo differentiation assays.
Main Results:
- Successfully derived two homogenous hESC sublines: SA002.5 (diploid) from SA002 (trisomy 13) and AS034.1.1 (reclone) from AS034.1.
- Both sublines express key undifferentiated hESC markers and exhibit high telomerase activity.
- Demonstrated pluripotency, forming derivatives of all three embryonic germ layers.
- SA002.5 provides a diploid counterpart to the trisomic SA002 line.
Conclusions:
- The novel homogenous hESC lines SA002.5 and AS034.1.1 are valuable for hESC-based applications.
- These lines are suitable for drug development and toxicity testing.
- The SA002/SA002.5 system offers a unique model for studying trisomy 13 pathologies.
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