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Pro-apoptotic effect of maize lipid transfer protein on mammalian mitochondria
Massimo Crimi1, Alessandra Astegno, Gianni Zoccatelli
1Department Scientific and Technologic, Faculty of Science, University of Verona, Strada le Grazie 15, 37134 Verona, Italy. massimo.crimi@univr.it
Abstract:
To assess the effect of lipids and lipid exchange in the pro-apoptotic release of cytochrome c, we investigated the ability of a plant lipid transfer protein (LTP) to initiate the apoptotic cascade at the mitochondrial level. The results show that maize LTP is able to induce cytochrome c release from the intermembrane space of mouse liver mitochondria without significant mitochondrial swelling, similarly to mouse full-length Bid. This effect is influenced by the presence of specific lipids, since addition of lysolipids like lysophosphatidylcholine strongly stimulates the LTP-induced release of cytochrome c while it is inhibited by removal of endogenous free lipids with a complete suppression of the LTP-induced release of cytochrome c. The results are discussed in light of the possible role of lipid exchange in apoptosis.
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