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Rapid chloride analysis using miniaturised isotachophoresis
Jeff E Prest1, Sara J Baldock, Peter R Fielden
1School of Chemical Engineering and Analytical Science, The University of Manchester, UK. j.prest@manchester.ac.uk
Journal of Chromatography. A
|December 6, 2005
Summary
A novel miniaturized device enables rapid isotachophoresis (ITP) analysis. This new system allows for quick chloride determination with high sensitivity, improving analytical speed and detection limits.
Area of Science:
- Analytical Chemistry
- Separation Science
- Microfluidics
Background:
- Isotachophoresis (ITP) is a powerful separation technique.
- Miniaturization of analytical devices offers advantages in speed and portability.
- Efficient detection of anions like chloride is crucial in various applications.
Purpose of the Study:
- To develop a miniaturized device for isotachophoresis (ITP).
- To create a novel electrolyte system for rapid chloride determination.
- To integrate an on-column detector for enhanced analysis.
Main Methods:
- Fabrication of a miniaturized separation device in poly(methyl methacrylate) with a 'double T' injection geometry.
- Development of a new electrolyte system using nitrate as the leading ion, with indium(III) as a complexing agent.
- Incorporation of an on-column conductivity detector for real-time monitoring.
Main Results:
- The miniaturized ITP device achieved chloride sample analysis in under 100 seconds.
- A limit of detection (LOD) of 2.2 mg l(-1) for chloride was calculated.
- The new electrolyte system demonstrated effective performance with the developed device.
Conclusions:
- The developed miniaturized ITP device and electrolyte system enable rapid and sensitive chloride analysis.
- This integrated system offers a promising solution for fast anion determination.
- The 'double T' injection geometry and on-column detection contribute to the device's efficiency.