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Related Experiment Videos

Amniotic fluid volume and composition in mouse pregnancy.

Cecilia Y Cheung1, Robert A Brace

  • 1Division of Perinatal Medicine, Department of Reproductive Medicine, University of California San Diego, La Jolla, California 92093-0802, USA. cycheung@ucsd.edu

Journal of the Society for Gynecologic Investigation
|December 6, 2005
PubMed
Summary

Amniotic fluid (AF) volume in mice increases significantly during mid-to-late gestation but sharply decreases before birth, unlike in humans. These dynamic changes in AF volume and composition offer insights into fetal development and potential genetic models.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Comparative Physiology

Background:

  • Amniotic fluid (AF) plays a crucial role in fetal development.
  • Understanding AF volume and composition dynamics is essential for assessing fetal well-being.
  • The pregnant mouse is a potential model for studying AF regulation.

Purpose of the Study:

  • To investigate simultaneous changes in amniotic fluid volume and composition throughout mouse gestation.
  • To establish a baseline for AF parameters in the pregnant mouse model.
  • To explore the feasibility of using genetically modified mice for AF research.

Main Methods:

  • AF was collected daily from embryonic days 9.5 to 18.5 in CB6F1 mice.
  • Measurements included AF volume, osmolality, and concentrations of key solutes (sodium, potassium, calcium, chloride, glucose, lactate).

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  • Statistical analysis utilized one-factor ANOVA.

    • Main Results:

    • AF volume increased from 18 microL at day 9.5 to a peak of 147 microL at days 15.5-16.5, then rapidly decreased to 17 microL by day 18.5.
    • AF osmolality remained stable, with a late rise before delivery.
    • Solute concentrations (Na+, Ca2+, glucose, K+, Cl-, lactate) exhibited distinct gestational patterns.

    Conclusions:

    • Mouse AF volume dynamics show similarities to humans in the mid-to-late gestational rise but differ in the pre-delivery decline.
    • Changes in AF volume correlate with osmolality and solute concentration shifts, similar to fetal sheep.
    • The mouse model is suitable for studying the molecular mechanisms regulating AF volume and composition.