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Updated: Aug 14, 2026
In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
Published on: May 12, 2023
Analysis of DNA intercalating drugs by TGGE
Patrik Danko1, Anton Kozák, Dusan Podhradský
1Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Safárik University, Kosice, Slovakia.
Abstract:
Temperature-gradient gel electrophoresis (TGGE) was used to study DNA-drug interactions. The results indicate that at least two classes of DNA intercalating drugs are distinguishable with respect to temperature increase: reversible and irreversible. The method offers an excellent means of visualizing the melting profile of an individual DNA topoisomer in the presence of DNA binding drugs. Our findings coincide with UV/VIS absorption spectroscopy data.
