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Related Experiment Videos

Mouse Ubc9 knockout: many path(way)s to ruin.

Michael R Kuehn1

  • 1Laboratory of Protein Dynamics and Signaling, National Cancer Institute, NCI-Frederick, Frederick, Maryland 21702, USA.

Developmental Cell
|December 6, 2005
PubMed
Summary
This summary is machine-generated.

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Researchers disrupted the Ubc9 gene, essential for SUMOylation, in mice. This disruption crippled nuclear functions in early embryonic cells, impacting key processes like chromosome segregation.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Background:

  • SUMOylation is a crucial post-translational modification regulating various cellular processes.
  • The SUMO-specific E2 enzyme Ubc9 plays a central role in the SUMO conjugation pathway.
  • Understanding Ubc9's function is vital for comprehending nuclear organization and cell proliferation.

Purpose of the Study:

  • To investigate the in vivo function of Ubc9 in early mouse embryonic development.
  • To elucidate the impact of abrogating the SUMO conjugation pathway on nuclear functions.
  • To identify the specific roles of Ubc9 in processes such as chromosome segregation and nucleo-cytoplasmic transport.

Main Methods:

  • Generation of a targeted germ line mutation in the mouse Ubc9 gene.

Related Experiment Videos

  • Analysis of embryonic lethality and developmental defects.
  • Assessment of nuclear organization, chromosome segregation, and nucleo-cytoplasmic transport in mutant cells.

    • Main Results:

    • Targeted mutation of Ubc9 abrogated the SUMO conjugation pathway in early embryos.
    • Loss of Ubc9 function led to widespread nuclear dysfunction in proliferating embryonic cells.
    • Defects were observed in nuclear organization, chromosome segregation, and Ran-driven nucleo-cytoplasmic transport.

    Conclusions:

    • Ubc9 is essential for normal nuclear function and embryonic development in mice.
    • The SUMO conjugation pathway, mediated by Ubc9, plays critical roles in maintaining genome stability and nuclear transport.
    • This study highlights the broad impact of Ubc9 on fundamental cellular processes during early development.