Related Experiment Video

Updated: Aug 14, 2026

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection
07:57

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

Published on: June 24, 2025

In vitro resistance to human platelet microbicidal protein among urethral staphylococcal and enterococcal isolates

I B Ivanov1

  • 1Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Orenburg, Russia. ubi@mail.orb.ru

Indian Journal of Medical Microbiology
|December 6, 2005
PubMed

Insights

Human platelet microbicidal protein (hPMP) shows reduced activity against common urethral pathogens isolated from chronic prostatitis (CP) patients. CP bacterial strains demonstrate significantly higher resistance to hPMP compared to non-CP strains.

Area of Science:

  • Microbiology
  • Urology
  • Immunology

Background:

  • Chronic prostatitis (CP) is often linked to bacterial infections.
  • Human platelet microbicidal protein (hPMP) possesses known antimicrobial properties.
  • Understanding pathogen resistance mechanisms is crucial for CP treatment.

Purpose of the Study:

  • To evaluate the in vitro antimicrobial activity of hPMP against common urethral pathogens.
  • To compare the susceptibility of bacterial isolates from CP patients versus non-CP individuals to hPMP.
  • To investigate the role of hPMP resistance in the pathogenesis of CP.

Main Methods:

  • In vitro susceptibility testing of bacterial isolates (Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus faecalis) to hPMP.
  • Serial dilution method used to determine hPMP minimum inhibitory concentrations.
  • Comparison of hPMP resistance profiles between clinical isolates from CP and non-CP patients.

Main Results:

  • CP isolates of coagulase-negative staphylococci showed significantly higher resistance to hPMP (91.3%) compared to non-CP isolates (5.88%).
  • A high percentage of Staphylococcus aureus (77.8%) and all Enterococcus faecalis CP strains were resistant to hPMP.
  • Most non-CP urethral isolates were susceptible to hPMP, while CP isolates exhibited significantly increased resistance across all tested species.

Conclusions:

  • Bacterial pathogens associated with chronic prostatitis display heightened resistance to human platelet microbicidal protein (hPMP).
  • The observed hPMP resistance in CP isolates may contribute to the understanding of chronic prostatitis pathogenesis.
  • Further research into hPMP's role and pathogen resistance is warranted for potential therapeutic strategies in CP.

More Related Videos

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP06:36

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

Published on: April 9, 2013

Related Concept Videos

Microbiota of the Urogenital Tract01:28

Microbiota of the Urogenital Tract

The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...