Related Experiment Video

Updated: Aug 14, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

p53 enhances the Delta-24 conditionally replicative adenovirus anti-glioma effect

P G Mitlianga1, C Sioka, G Vartholomatos

  • 1NeuroSurgical Institute, University of Ioannina, Greece. pmitliag@cc.uoi.gr

Oncology Reports
|December 6, 2005
PubMed

Insights

Combining Ad5Delta24 adenovirus with p53 gene therapy shows enhanced cell death in glioma cells. This dual approach, targeting the p16/Rb/E2F pathway and apoptosis, offers a promising new strategy for glioma treatment.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Glioma is a challenging brain tumor with limited treatment options.
  • The conditionally replicative adenovirus Ad5Delta24 selectively targets glioma cells with defective p16/Rb/E2F pathways.
  • The p53 protein acts as an apoptotic factor in glioma cells.

Purpose of the Study:

  • To investigate the combined therapeutic effect of Ad5Delta24 adenovirus and exogenous p53 delivery in glioma cells.
  • To determine if simultaneous administration enhances anti-tumor activity.

Main Methods:

  • Glioma cells were infected with low doses of Ad5CMV-p53 and Ad5Delta24 adenoviruses.
  • Cell death was assessed using flow cytometry.
  • The mechanism of cell death (apoptosis and lysis) was analyzed.

Main Results:

  • The combination of Ad5CMV-p53 and Ad5Delta24 induced an additive effect on glioma cell death.
  • The enhanced cell death was independent of the cells' p53 status.
  • Flow cytometry confirmed that the anti-tumor effect resulted from a combination of apoptosis and cell lysis.

Conclusions:

  • Ad5CMV-p53 significantly enhances the oncolytic effect of Ad5Delta24 adenovirus.
  • The combination of Ad5Delta24 and Ad5CMV-p53 represents a potential therapeutic strategy for treating gliomas.

More Related Videos

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...