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P2Y isoforms operative in mouse taste cells.

Marina F Bystrova1, Yulia E Yatzenko, Ilya V Fedorov

  • 1Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia. staskolesnikov@yahoo.com

Cell and Tissue Research
|December 6, 2005
PubMed
Summary

Mouse taste cells utilize P2Y receptors for sensing nucleotides like ATP and UTP. Studies confirm P2Y2 and P2Y4 receptors are key players in taste cell responses to these compounds.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Sensory Biology

Background:

  • Taste cells express P2Y receptors involved in intracellular calcium signaling.
  • Previous research suggests a role for purinergic signaling in taste perception.

Purpose of the Study:

  • To investigate the specific P2Y receptor subtypes expressed in mouse taste cells.
  • To determine the role of these receptors in mediating taste cell responses to extracellular nucleotides.

Main Methods:

  • Functional assays measuring intracellular calcium mobilization.
  • Reverse transcription-polymerase chain reaction (RT-PCR) for gene expression analysis.
  • Immunohistochemistry to localize receptor protein expression in taste buds.

Main Results:

Related Experiment Videos

  • ATP and UTP induced equipotent intracellular calcium mobilization, suggesting shared receptor involvement.
  • RT-PCR and immunohistochemistry confirmed the expression of P2Y2 and P2Y4 receptors in mouse taste bud cells.
  • P2Y1 and P2Y6 receptor transcripts were also detected, correlating with ADP and UDP responsiveness.

Conclusions:

  • P2Y2 and P2Y4 receptors are predominantly expressed in mouse taste cells.
  • These receptors play a significant role in mediating taste cell responses to ATP and UTP.
  • Purinergic signaling via specific P2Y receptor subtypes contributes to taste perception.