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Updated: Aug 14, 2026

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Published on: December 5, 2016
Engineering the chloroplast encoded proteins of chlamydomonas
1Department of Plant Biology, Ohio State University, Columbus, OH, 43210, USA.
Abstract:
Over a decade ago (1988), John Boynton and colleagues successfully transformed the chloroplast genome of chlamydomonas for the first time by complementation of a chloroplast deletion mutant. Since the first demonstration of chloroplast transformation the function and structure of many chloroplast encoded subunits of the photosynthetic apparatus has been characterized by site-directed mutagenesis. With the completion of the sequencing of the Chlamydomonas chloroplast genome the genetic tools are now in hand to characterize structure-function relationships for each of the chloroplast-encoded proteins of the photosynthetic apparatus.
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