Related Experiment Video
Updated: Aug 14, 2026

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
Published on: February 16, 2015
Salt is necessary for nucleosomal DNA fragmentation induced by caspase
Bo-Eun Kim1, Yun-Hee Lee, Changhyun Seong
1Research Center for Proteineous Materials (RCPM) and Department of Bio-Materials Engineering, Chosun University, 375 Seosuk-Dong, Dong-Ku, Gwangju 501-759, Republic of Korea.
Abstract:
For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).
Related Concept Videos
Caspases
DNA Isolation
The Intrinsic Apoptotic Pathway

