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Updated: Aug 14, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
[Assessment of antioxidative enzymes activity and intensification of lipids peroxidation in asthmatic patients]
Barbara Rogala1, Renata Polaniak, Alicja Grzanka
1Slaskiej Akademii Medycznej w Katowicach, Katedra i Klinika Chorób Wewnetrznych, Alergologii i Immunologii Klinicznej w Zabrzu.
Abstract:
Chronic immunoallergic inflammatory reaction plays a key role in pathogenesis of asthma. Importance of free oxygen radicals in mechanism of chronic inflammatory process has been proved. Activity of two antioxidative enzymes: isoenzymes of superoxide dismutase (SOD), glutathione peroxidase (POX), and concentration of malondialdehyde (MDA) in serum and erythrocytes in asthmatics during exacerbation and improvement of disease were assessed. Disturbances in oxidative system in asthmatic patients have been observed. Lack of significant differences in antioxidative indexes between a period of exacerbation, not complicated by infection and a period of improvement indicates a pathophysiological role of chronic oxygen stress in asthma. It has been also shown that bacterial infection disturbs efficiency of antioxidative mechanisms.
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