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Updated: Aug 14, 2026
Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
Published on: May 24, 2018
[UCPs and PPARgamma2 mRNA in diet induced obesity resistant rats]
Nian-Hong Yang1, Chong-Jian Wang, Ming-Jia Xu
1Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Objective:
To explore the expression of uncoupling proteins (UCP) and peroxisome proliferator activated receptorgamma2 (PPARgamma2) mRNA in white adipose of high-fat diet induced obesity resistant rats.
Methods:
Thirty-six female SD rats were randomly divided into high-fat diet group (n = 27) and chow fed control group (n = 9), and given either high-fat diet or chow for thirteen weeks. Then the high-fat diet group was subdivided into Dietary Induced Obesity (DIO) and Dietary Induced Obesity Resistant (DIO-R) group according to the final body weight. Basic parameters, fasting blood glucose (FBG), blood lipid levels were measured and compared. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the expression of UCP2, UCP3, and PPARgamma2 mRNA in white adipose.
Results:
Adipose deposits, body fat percent, TC, and TG conceration of both DIO-R and DIO groups were significantly higher than those in control group, while body weight, body fat percent and TG level were significantly lower in DIO-R than DIO rats. The expression of UCP2 and UCP3 mRNA in white adipose of DIO-R rats were significantly higher than those of DIO and control group. The expression of PPARgamma2 mRNA in white adipose of DIO-R group was significantly lower than that in DIO group.
Conclusion:
High-fat diet induced obesity resistance were closely associated with the increased UCP2, UCP3, and decreased PPARgamma2 mRNA in white adipose.
