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Fluorescence and dynamic light scattering studies of niosomes-membrane mimetic systems

Agnishwar Girigoswami1, Susmita Das, Swati De

  • 1Department of Chemistry, University of Kalyani, Kalyani-741235, India.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|December 7, 2005
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...