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Comparative analysis of thyroxine distribution in ascidian larvae
Paolo D'Agati1, Matteo Cammarata
1Department of Animal Biology, University of Palermo, Via Archirafi 18, 90123, Palermo, Italy.
Cell and Tissue Research
|December 7, 2005
Summary
Thyroid hormones, specifically L-thyroxine (T4), are present in ascidian larvae and localized in mesenchymal cells. This discovery suggests a potential role for thyroid hormones in controlling ascidian metamorphosis.
Area of Science:
- Marine Biology
- Endocrinology
- Developmental Biology
Background:
- The ascidian endostyle is known for iodine concentration and thyroid hormone biosynthesis.
- Recent findings indicate thyroid hormones are present in ascidian mesenchymal cells.
Purpose of the Study:
- To investigate the presence and localization of L-thyroxine (T4) in four ascidian species larvae.
- To explore the involvement of T4 in ascidian metamorphosis.
Main Methods:
- In vivo larval treatment with T4 and thiourea.
- In vitro confirmation using dot blotting, radioimmunoassay, and immunoperoxidase staining.
Main Results:
- T4 was detected in the mesenchymal cells of Ascidia malaca, Ascidiella aspersa, Phallusia mamillata, and Ciona intestinalis larvae.
- Localization included the body cavity, under adhesive papillae, and around the intestine.
- Presence of T4 in mesenchymal cells may relate to blood cells, musculature, and heart tissue differentiation.
Conclusions:
- Thyroid hormones (T4) are present in ascidian larvae.
- Mesenchymal cell localization suggests a role in tissue differentiation.
- Results indicate thyroid hormones may control ascidian metamorphosis.