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Fitness for purpose--ochratoxin A analytical developments.

A Visconti¹, A De Girolamo

¹Institute of Sciences of Food Production, CNR, ISPA, Bari, Italy. angelo.visconti@ispa.cnr.it

Food Additives and Contaminants

|December 8, 2005

Summary

Ensuring laboratory quality assurance for ochratoxin A (OTA) analysis requires validated methods and proficiency testing. Various techniques, from chromatography to novel biosensors, exist for accurate OTA detection in foods and beverages.

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Area of Science:

Food safety and analytical chemistry.
Mycotoxin analysis and quality assurance.

Background:

Ochratoxin A (OTA) is a significant mycotoxin found in various food products.
Accurate and reliable analysis of OTA is crucial for consumer safety and regulatory compliance.

Purpose of the Study:

To review and discuss current and emerging analytical methodologies for ochratoxin A (OTA) determination.
To highlight essential elements for laboratory quality assurance in OTA analysis.

Main Methods:

Review of established methods: liquid chromatography with fluorescence detection (LC-FD), enzyme-linked immunosorbent assays (ELISA).
Exploration of novel technologies: electrochemical immunosensors, biosensors, lateral flow devices.
Discussion of advanced techniques: liquid chromatography-mass spectrometry (LC-MS), molecularly imprinted polymers (MIPs).

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Main Results:

LC-FD methods, often with immunoaffinity or solid-phase extraction, are validated standards for OTA determination.
ELISA kits serve as common screening tools for OTA in food.
Emerging immunochemical methods and LC-MS offer rapid analysis and multi-mycotoxin capabilities.

Conclusions:

Laboratory accreditation, validated methods, and proficiency testing are vital for reliable OTA analysis.
Continuous validation of new immunochemical methods and kits is necessary.
Alternative cleanup techniques like MIPs show promise for cost-effective OTA analysis.