Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural and functional properties of mouse-human chimeric IgD.

S U Shin1, C F Wei, A R Amin

  • 1Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.

Human Antibodies and Hybridomas
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diastolic dysfunction grading, echocardiographic and electrocardiogram findings in 50 patients with apical hypertrophic cardiomyopathy.

The Medical journal of Malaysia·2020
Same author

Possible mechanism for down-regulation of autoantibody production by auto-anti-idiotype.

Immunology today·2014
Same author

SITES OF FORMATION OF IMMUNE GLOBULINS AND OF A COMPONENT OF C'(3): I. A NEW TECHNIQUE FOR THE DEMONSTRATION OF THE SYNTHESIS OF INDIVIDUAL SERUM PROTEINS BY TISSUES IN VITRO.

The Journal of experimental medicine·2009
Same author

SITES OF FORMATION OF IMMUNE GLOBULINS AND OF A COMPONENT OF C'(3): II. PRODUCTION OF IMMUNOELECTROPHORETICALLY IDENTIFIED SERUM PROTEINS BY HUMAN AND MONKEY TISSUES IN VITRO.

The Journal of experimental medicine·2009
Same author

"A system biology" approach to bioinformatics and functional genomics in complex human diseases: arthritis.

Current issues in molecular biology·2002
Same author

[Intravenous infection of C. albicans induces apoptosis of cortical thymocytes in mice].

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University·2002

Researchers created a mouse-human chimeric immunoglobulin D (IgD) that binds to the IgD receptor on T cells. This chimeric IgD, specific for the hapten dansyl, mimics natural IgD function and is crucial for T cell activation.

Area of Science:

  • Immunology
  • Molecular Biology
  • Glycobiology

Background:

  • Immunoglobulin D (IgD) is a key B cell receptor and plays a role in T cell activation.
  • Understanding IgD structure-function relationships is crucial for immunological research.

Purpose of the Study:

  • To construct and characterize a mouse-human chimeric IgD molecule.
  • To investigate the role of glycosylation in IgD assembly and function.
  • To assess the chimeric IgD's ability to interact with the IgD receptor (IgD-R) on T cells.

Main Methods:

  • Gene construction of chimeric IgD using murine variable and human constant regions.
  • Expression and secretion of chimeric IgD (H2L2 molecule).
  • Analysis of N-linked and O-linked glycosylation.

Related Experiment Videos

  • Tunicamycin inhibition of N-linked glycosylation.
  • Functional assays measuring IgD-R binding and upregulation on human T cells.

    • Main Results:

    • Successfully synthesized and secreted a chimeric IgD specific for the hapten dansyl.
    • Chimeric IgD heavy chain features N-linked and O-linked glycosylation, with N-linked glycosylation occurring co- and post-translationally.
    • Inhibition of N-linked glycosylation disrupted assembly and secretion.
    • Chimeric IgD effectively binds and upregulates IgD-R on T cells, similar to natural IgD.
    • Cross-linking with dansyl-BSA was necessary for soluble chimeric IgD to induce IgD-R upregulation.

    Conclusions:

    • Chimeric IgD provides a valuable tool for studying IgD structure and function.
    • Glycosylation is essential for proper IgD assembly, secretion, and receptor interaction.
    • Chimeric IgD successfully mimics natural IgD's interaction with the IgD receptor, offering insights into T cell modulation.