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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
Published on: October 30, 2016
Clonal nature of Campylobacter fetus as defined by multilocus sequence typing
Marcel A P van Bergen1, Kate E Dingle, Martin C J Maiden
1Animal Sciences Group, Division of Infectious Diseases, P.O. Box 65, 8200 AB Lelystad, The Netherlands.
Journal of Clinical Microbiology
|December 8, 2005
Summary
Multilocus sequence typing (MLST) reveals Campylobacter fetus is genetically homogeneous, with subspecies closely related. MLST data confirms genetic stability in mammalian Campylobacter fetus, likely from a single ancestral clone.
Area of Science:
- Microbiology
- Genetics
- Veterinary Medicine
Background:
- Campylobacter fetus subspecies differentiation is challenging despite distinct host preferences.
- Existing typing methods yield varied classifications for C. fetus isolates.
- Understanding evolutionary relationships is key to assessing typing method value.
Purpose of the Study:
- Develop a multilocus sequence typing (MLST) scheme for Campylobacter fetus.
- Assess the genetic diversity and evolutionary relationships within C. fetus.
- Correlate MLST data with previous typing methods like AFLP analysis.
Main Methods:
- Developed and applied a multilocus sequence typing (MLST) scheme to 140 C. fetus isolates.
- Compared MLST results with previous amplified fragment length polymorphism (AFLP) typing.
- Analyzed genetic diversity by identifying sequence types (STs) and variable sites.
Main Results:
- Identified 14 distinct sequence types (STs) within C. fetus.
- Observed low inter-ST genetic diversity, indicating overall genetic homogeneity.
- Found C. fetus subspecies to be genetically very similar, with ST-4 linked to the 'bovine' clone (C. fetus subsp. venerealis).
- C. fetus subsp. fetus isolates showed greater ST diversity correlating with epidemiology.
- Congruence observed between subspecies, sap type, and ST.
Conclusions:
- MLST confirms Campylobacter fetus is genetically stable, likely originating from a single ancestral clone.
- The developed MLST scheme is a valuable tool for typing C. fetus isolates.
- Genetic homogeneity supports the introduction of a single clone into mammalian niches.
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