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Related Experiment Videos

Gene regulation by transmembrane signaling.

Volkmar Braun1, Susanne Mahren, Annette Sauter

  • 1Microbiology/Membrane Physiology, University of Tübingen, Auf der Morgenstelle 28, Tübingen 72096, Germany. volkmar.braun@mikrobio.uni-tuebingen.de

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
|December 8, 2005
PubMed
Summary

Ferric citrate transport in E. coli involves a novel signaling cascade. The inducer binds an outer membrane protein, triggering a signal across cell membranes to regulate gene transcription without entering the cell.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Escherichia coli K-12 utilizes ferric citrate for iron uptake.
  • Transcriptional regulation of ferric citrate transport genes is complex.

Purpose of the Study:

  • To elucidate the novel mechanism of ferric citrate transport gene regulation in E. coli.
  • To identify the key proteins and signaling pathways involved.

Main Methods:

  • Investigated ferric citrate transport genes in E. coli K-12.
  • Analyzed the roles of FecA, FecR, and FecI proteins in signal transduction.
  • Examined the influence of iron availability and Fur repressor activity.

Main Results:

  • Ferric citrate acts as an extracellular signal, binding to outer membrane protein FecA.

Related Experiment Videos

  • A transmembrane signaling cascade involving FecR and FecI activates transcription of fecABCDE genes.
  • Regulation is linked to iron availability via the Fur repressor controlling fecI and fecR gene expression.

    • Conclusions:

    • Discovered a novel tripartite transmembrane signaling system for ferric citrate transport regulation.
    • This system exemplifies a broader class of gene regulation involving signal transmission across multiple cellular compartments.