Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A well-mixed, polymer-based microbioreactor with integrated optical measurements.

Zhiyu Zhang1, Nicolas Szita, Paolo Boccazzi

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Mass. Ave., 66-566, Cambridge, Massachusetts 02139, USA.

Biotechnology and Bioengineering
|December 8, 2005
PubMed
Summary

This study presents a novel microbioreactor for microbial cell culture, enabling precise control and online monitoring. The device demonstrates effective cell growth for Escherichia coli and Saccharomyces cerevisiae, offering a reproducible and flexible alternative to traditional methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathway-Aware Template-Based Retrosynthesis.

Journal of chemical information and modeling·2026
Same author

Continuous production of recombinant adeno-associated virus in the insect cell/baculovirus expression vector system.

Molecular therapy. Advances·2026
Same author

Rethinking viral vector quantification: a microfluidic approach to standardised functional titre assays.

Frontiers in bioengineering and biotechnology·2026
Same author

Artificial intelligence-assisted mining of polyethylene terephthalate hydrolases.

New biotechnology·2026
Same author

Water-in-oil emulsion fermentation as a platform for poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) production from hydrophobic feedstocks.

Bioresource technology·2026
Same author

Machine Learning and Autonomous Systems for Accelerated Synthesis.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026

Area of Science:

  • Biotechnology and Bioengineering
  • Microfluidics and Lab-on-a-Chip Devices
  • Microbial Cultivation and Bioprocessing

Background:

  • Traditional microbial cultivation methods often lack precise control and real-time monitoring capabilities.
  • There is a need for miniaturized, integrated systems for efficient microbial cell culture.
  • Poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) are suitable materials for microfluidic device fabrication.

Purpose of the Study:

  • To design and fabricate a 150 microL microbioreactor for microbial cell cultivation.
  • To integrate online monitoring sensors for pH, dissolved oxygen, and cell density.
  • To evaluate the mixing and oxygenation performance and demonstrate cell growth capabilities.

Main Methods:

  • Fabrication of the microbioreactor using poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS).

Related Experiment Videos

  • Integration of magnetic stir bar for mixing and fluorescent sensors for real-time measurements.
  • Use of optical transmission for cell density monitoring and computational fluid dynamics (CFD) for mixing analysis.
  • Measurement of oxygen transfer coefficients (KLa) at varying stirring speeds.

    • Main Results:

    • The microbioreactor achieved oxygenation values (KLa) ranging from 20-75/h with stirring speeds of 200-800 rpm.
    • Demonstrated successful cultivation and growth of Escherichia coli, benchmarked against conventional methods.
    • Showcased reproducible batch cultures of Saccharomyces cerevisiae, highlighting system flexibility.

    Conclusions:

    • The developed microbioreactor offers a robust platform for microbial cell culture with integrated monitoring.
    • The system provides efficient mixing and oxygenation, crucial for microbial metabolism.
    • This technology presents a promising, reproducible, and flexible alternative for microbial bioprocessing.