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Updated: Aug 12, 2026
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Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Tyrosine kinases and their interactions with signalling proteins
1Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Current Opinion in Genetics & Development
|February 1, 1992
Summary
Researchers identified Trk and Met tyrosine kinases as receptors for nerve growth factor and hepatocyte growth factor, respectively. Activated receptors signal by associating with SH2-containing proteins, advancing understanding of signal transduction pathways.
Area of Science:
- Molecular biology
- Cell signaling
- Biochemistry
Background:
- Receptor protein-tyrosine kinases (RPTKs) play crucial roles in cellular processes.
- Understanding the specific RPTKs and their downstream signaling is vital for biological research.
Purpose of the Study:
- To identify the specific receptor tyrosine kinases involved in nerve growth factor and hepatocyte growth factor signaling.
- To elucidate the mechanism of intracellular signal transduction initiated by these activated receptors.
Main Methods:
- Identification of receptor tyrosine kinases.
- Analysis of protein-protein interactions in signal transduction pathways.
Main Results:
- The Trk receptor tyrosine kinase has been identified as the receptor for nerve growth factor.
- The Met receptor tyrosine kinase has been identified as the receptor for hepatocyte growth factor.
- Activated Trk and Met receptors associate with SH2-containing cytoplasmic signaling proteins to mediate downstream effects.
Conclusions:
- Nerve growth factor and hepatocyte growth factor signal through the Trk and Met receptor tyrosine kinases, respectively.
- The recruitment of SH2-containing proteins to autophosphorylated receptors is a key step in initiating intracellular signaling cascades.
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