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Updated: Aug 4, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Published on: September 9, 2010
[Establishing a method to detect the DNA damage of N-ras gene]
Chao-Long Rao1, Zun-Zhen Zhang, Zheng-Chang Heng
1Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
Applying RDPCR to detect the DNA damage of N-ras gene in human.
Methods:
Single-primer PCR was used to prepare single strand (ss) probes of extron 1 of N-ras gene in human. The genomic DNA was digested completely by restriction endonuclease, then amplified by RDPCR and detected by Southern hybridization with the probe.
Results:
The ss probes were successfully prepared by single-primer PCR. The hybridized bands were clearly seen in the expected migration positions.
Conclusion:
The result shows that the method to detect the damaged position of N-ras gene has been established, which would be helpful to further studies on chemical carcinogenesis and on the prevention of tumor.
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