[Glucose-fatty acids cycle in cobalt chloride-induced oxidative stress in rats]

It was found that the glucose-fatty acids cycle functioned under the oxidative stress, caused by injection of cobalt chloride solution in albino rats. This cycle promoted the adaptation of metabolism and rehabilitated the homeostasis under extreme conditions. Its functioning was regulated by prolonged (during 2-24 hours) rise in activity of amino acids catabolism enzymes (e.g. tyrosine aminotransferase, arginase) and activation of glyconeogenesis after the mobilisation of liver glycogen. This contributed to increase in glucose and free fatty acids contents in blood. The latter is additionally provided by lipid mobilisation under stress. Tyrosine aminotransferase activation occurred both on the transcription level and by enabling of other mechanisms, which probably concerned the stabilisation of this enzyme. Preliminary injection of alpha-tocopherol in vivo significantly decreased the rise in tyrosine aminotransferase and arginase activities and the rate of erythrocyte hemolysis but did not disable them in full. This made evident that in regulation of the glucose-fatty acids cycle not only active metabolites of oxygen but also Co ions were directly enabled.