Related Experiment Video
Updated: Aug 14, 2026
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
Published on: May 11, 2021
[A three-dimension in vitro model for angiogenesis of hemangioma]
Cheng-Hong Jiang1, Fu-Lian Zhuang, Ba-Rui Huang
1The Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Objective:
To create a three dimension (3D) in vitro model for angiogenesis of hemangioma.
Method:
The fragment of hemangioma specimen was embedded in fibrin gel to set up the three-dimension (3D) in vitro model for angiogenesis of hemangioma.
Result:
In the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being, then tending to be stationary. The compact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electron microscopy.
Conclusion:
This model helps to study the mechanism of hemangioma angiogenesis and investigate the drugs of anti-angiogenesis.
Related Concept Videos
01:10Mechanism of Angiogenesis
01:24Regulation of Angiogenesis and Blood Supply