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Updated: Aug 14, 2026

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
Published on: September 22, 2020
Structure and nucleotide polymorphisms in pig uncoupling protein 2 and 3 genes
Hanjie Li1, Yanhua Li, Xingbo Zhao
1College of Animal Science and Technology, State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, P.R. China.
Abstract:
Uncoupling proteins (UCPs) are mitochondrial membrane transporters, acting as an uncoupler in oxidative phosphorylation. In this study, we designed 11 primer sets based on the human and mouse UCP2, UCP3 sequences and successfully amplified full regions of porcine UCP2 and UCP3 by polymerase chain reactions (PCR). Comparison of the UCP2 and UCP3 genic structures revealed a highly conservative region was putatively presented, showing the second transmembrane domain may be the UCPs' cardinal function region. Altogether 23 nucleotide polymorphisms of UCP2 and UCP3 genes were discovered in Yorkshire, Wuzhishan, and Lepinghua pigs. These polymorphisms included 3 missense mutations, 16 intronic substitutions, and 4 intronic deletions. The substitution of Ala-55-Val in UCP2 is actually the most common mutation in human. We also calculated genotypic frequencies of five polymorphisms in three pig breeds.
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