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Updated: Aug 14, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
Identification of class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass
Kenneth L Johnson1, Inna G Ovsyannikova, Gregory A Poland
1Mayo Proteomics Research Center, Mayo Vaccine Research Group, Program in Translational Immunovirology and Biodefense, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Abstract:
Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.
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