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Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood
Andrzej K Drukier1, Natascha Ossetrova, Elena Schors
1BioTraces Inc., Herndon, Virginia, USA.
Abstract:
Multiphoton-detection methods that detect as little as 1000 atoms of (125)I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNFalpha, VEGF, IL-1beta, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.
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