Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Influence of different cell extraction methods on cytometric features.

F B Thunnissen1, H Perdaen, J Forrest

  • 1Department of Pathology, University of Limburg, Maastricht, The Netherlands.

Cytometry
|January 1, 1992
PubMed
Summary

Cell extraction methods minimally impact DNA content measurements but significantly affect nuclear area and texture features. Reliable DNA content analysis requires approximately 25-30 nuclei per sample.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real World Multi-centre UK Review of Nivolumab Monotherapy in Metastatic Endometrial Cancer With Mismatch Repair Deficiency During COVID-19.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Induction chemotherapy followed by chemoradiation in locally advanced cervical cancer: Quality of life outcomes of the GCIG INTERLACE trial.

European journal of cancer (Oxford, England : 1990)·2025
Same author

The Royal College of Radiologists National Vulvar Cancer Audit.

Clinical oncology (Royal College of Radiologists (Great Britain))·2024
Same author

Defining the Optimal Treatment Strategy in Patients With Uterine Serous Carcinoma.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022
Same author

Risk factors associated with excessive negative energy balance in commercial United Kingdom dairy herds.

Veterinary journal (London, England : 1997)·2019
Same author

Prevalence of excessive negative energy balance in commercial United Kingdom dairy herds.

Veterinary journal (London, England : 1997)·2019

Area of Science:

  • Cytometry
  • Histology
  • Biomedical imaging

Background:

  • Accurate nuclear DNA content analysis is crucial for cell cycle studies and disease diagnosis.
  • Standardization of cell preparation techniques is essential for reproducible results in quantitative cytology.

Purpose of the Study:

  • To evaluate how different cell extraction procedures influence nuclear DNA content (IOD), nuclear area, and texture features.
  • To determine the optimal number of nuclei for reliable DNA content measurements.
  • To assess the impact of sampling methods on ploidy ratio calculations.

Main Methods:

  • Feulgen staining of nuclei from human liver fine-needle aspirates, imprints, and suspensions.
  • Measurement of relative DNA content (IOD), nuclear area, and texture features.

Related Experiment Videos

  • Calculation of progressive mean IOD and ploidy ratios (tetraploid/diploid, octaploid/diploid).

    • Main Results:

    • Progressive mean IOD stabilized after measuring 25-30 nuclei.
    • Diploid nuclei IOD showed minor variations (average coefficient of variation ~5%) across methods.
    • Ploidy ratios (tetraploid/diploid: 1.9-2.1, octaploid/diploid: 3.7-4.3) remained consistent.
    • Nuclear area and texture features significantly differed (p<0.01) between sampling methods.

    Conclusions:

    • Cell extraction procedures have minimal effect on DNA-related IOD measurements.
    • Nuclear area and texture feature analysis are highly dependent on the chosen cell extraction method.
    • Standardization of cell preparation is critical for texture and area-based nuclear feature analysis.