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Published on: March 8, 2020
Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi
Andries S Bareetseng1, Johan L F Kock, Carolina H Pohl
1Department of Microbial, Biochemical and Food Biotechnology, UNESCO MIRCEN: Industrial Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, South Africa.
Abstract:
The distribution of 3-hydroxy oxylipins in Saturnispora saitoi was mapped using immunofluorescence microscopy. Fluorescence was observed on aggregating ascospores, indicating the presence of 3-hydroxy oxylipins on the surface or between ascospores. The oxylipin was identified as 3-hydroxy 9:1 using gas chromatography mass spectrometry. Furthermore, ultrastructural studies using scanning and transmission electron microscopy on ascospores revealed a clear equatorial ledge surrounding oval-shaped ascospores.
