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Related Experiment Videos

Prosomes and their multicatalytic proteinase activity.

H G Nothwang1, O Coux, F Bey

  • 1Institut Jacques Monod, CNRS, Université Paris 7, France.

European Journal of Biochemistry
|July 15, 1992
PubMed
Summary

Prosomes, identified as messenger ribonucleoprotein (mRNP) complexes, possess protease activity, functioning as multicatalytic proteinase complexes (MCPs). These prosome-MCP particles are implicated in regulating protein levels within cells.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Prosomes were initially characterized as messenger ribonucleoprotein (mRNA-associated RNP) particles.
  • They are recognized as subcomponents of repressed messenger ribonucleoprotein (mRNP) complexes.

Purpose of the Study:

  • To investigate the protease activity of prosomes isolated from translationally inactive mRNP.
  • To determine if this protease activity is identical to the multicatalytic proteinase complex (MCP, or proteasome).

Main Methods:

  • Isolation of prosomes from avian erythroblast repressed non-globin mRNP.
  • Sucrose gradient sedimentation analysis following RNase or detergent treatment.
  • Identification of prosomes using monoclonal antibodies.
  • Immunoprecipitation of protease activity and isolation of associated small RNAs.

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  • Cesium chloride (CsCl) gradient centrifugation to assess colocalization.

    • Main Results:

    • Prosomes isolated from translationally inactive mRNP exhibit protease activity identical to MCP.
    • MCP activity associated with repressed mRNP can be shifted to the 19-S sedimentation zone characteristic of prosomes.
    • Small RNAs (80-120 nucleotides) are associated with the prosome-MCP complex.
    • MCP activity, prosomal proteins, and prosomal RNA colocalize on CsCl gradients.

    Conclusions:

    • The multicatalytic proteinase complex (MCP) activity is an intrinsic property of prosomes.
    • Prosomes are ribonucleoprotein (RNP) particles and subcomplexes of in vivo untranslated mRNP.
    • Prosome-MCP particles may play a role in maintaining cellular protein homeostasis.