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Use of trypan blue for penetrating keratoplasty
Jonathan C P Roos1, Malcolm G Kerr Muir
1Department of Medicine, Addenbrooke's Hospital, University of Cambridge, Cambridge, United Kingdom. jcpr2@cam.ac.uk
Journal of Cataract and Refractive Surgery
|December 13, 2005
Abstract:
Use of trypan blue for penetrating keratoplasty was developed to facilitate the procedure. Trypan blue is injected before and after the addition of 0.25 mL of an ophthalmic viscosurgical device (OVD), sodium hyaluronate, to stain the internal and external cut edge of the cornea as well as the OVD, enabling the surgeon to improve visualization of the incision and suture depth, improve alignment of host and donor tissues, and ensure that all OVD is removed.

