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Novel model for study of human sperm chemotaxis
C Villanueva-Díaz1, J Arias-Martínez, H Bustos-López
1Instituto Nacional de Perinatología, México City, México.
Objective:
To develop and test an adequate method for the study of sperm chemotaxis.
Design:
A model based on a double-chamber design and a matrix of 0.5% hyaluronic acid was used to test the response of human sperm to chemotactic signals from follicular fluid (FF).
Results:
A plateau of migration was reached at 10 minutes of incubation; under this condition, 70% of sperm migrated toward chambers with FF, less than 20% to the opposite side, and the rest remained at the site of application. Intra-assay and interassay coefficients of variation using pools of FFs were 17.2% and 23%, respectively.
Conclusion:
These data confirm previous findings and support the use of this novel model for the study of chemotaxis on human gametes.