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CD43 functions as a ligand for E-Selectin on activated T cells
Masanori Matsumoto1, Kazuyuki Atarashi, Eiji Umemoto
1Laboratory of Immunodynamics, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, Japan.
Journal of Immunology (Baltimore, Md. : 1950)
|December 13, 2005
Summary
CD43, a glycoprotein, acts as an E-selectin ligand on T cells, mediating cell rolling on inflamed sites. This finding reveals a new mechanism for leukocyte migration and inflammation.
Area of Science:
- Immunology
- Cell Biology
- Glycobiology
Background:
- E-selectin mediates leukocyte rolling on endothelial cells via E-selectin ligands.
- P-selectin glycoprotein ligand-1 (PSGL-1) is a known E-selectin ligand on Th1 cells.
- The identity of other E-selectin ligands on T cells remains largely unknown.
Purpose of the Study:
- To identify novel E-selectin ligands on activated T cells.
- To elucidate the molecular nature and function of these ligands.
- To understand their role in T cell migration to inflamed tissues.
Main Methods:
- Precipitation of E-selectin ligands using E-selectin-IgG chimera from mouse Th1 cells.
- Enzymatic cleavage and antibody-based characterization of precipitated proteins.
- Generation of CD43-IgG chimera and assessment of E-selectin-dependent cell rolling under flow conditions.
Main Results:
- A 130-kDa glycoprotein, identified as CD43, was precipitated by E-selectin-IgG.
- CD43 binding to E-selectin required sialic acid and specific glycosyltransferase modifications.
- E-selectin-dependent rolling of T cells on CD43 was demonstrated under flow conditions.
Conclusions:
- CD43, when appropriately glycosylated, functions as an E-selectin ligand.
- This identifies CD43 as a key mediator of activated T cell migration to inflamed sites.
- The findings provide new insights into leukocyte trafficking and inflammatory responses.