Related Experiment Video
Updated: Aug 14, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Studies on interaction of norfloxacin, Cu2+, and DNA by spectral methods
Gongwu Song1, Qiujun Yan, Yu He
1Faculty of Chemistry and Material Science, Hubei University, Wuhan, 430062, People's Republic of China. songgw@hubu.edu.cn
Abstract:
The interactions of norfloxacin (NFA), DNA, and Cu2+ are studied by fluorescence and UV-spectra method. According to the experimental results, it can be concluded that NFA can form a steady binary complex with Cu2+. There is a linear relationship between the Fluorescence intensity of the norfloxacin-Cu2+-DNA system and the concentration of DNA. And when the concentration of the NFA is 1.95x10(-5) mol L-1, they possess a good linearity in the concentration of DNA ranged from 4.7x10(-6) to 2.8x10(-5) mol L-1. It is a good method due to the high sensitivity and selectivity.

