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Updated: Aug 14, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
[Purification and properties of anitibiotic from Burkholderia cepacia CF-66]
Chun-shan Quan1, Wei Zheng, Zhi-ming Cao
1College of Life Science, Dalian Nationalities University, China. mikyeken@dlnu.edu.cn
Abstract:
Burkholderia cepacia CF-66 isolated from compost showed antifungal activity against plant pathogens such as Rhizoctomia solani and some other fungi. CF66I produced by Burkholderia cepacia CF-66 was separated by gel chromatography using Sephadex-75pg and Sephacryl S-100 column. CF66I has very high degree of stability of antifungal activity under higher temperature and alkaline conditions. Some organic solvents could improve its activity at low concentration. The research for the structure of CF66I find that it is a compound with amide bonds and its main structural units is (CH2CH2O)n.
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