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Corneal function after storage in dexsol or optisol.

R J Walkenbach1, F Boney, G S Ye

  • 1Missouri Lions Eye Research Foundation, University of Missouri School of Medicine, Columbia 65201.

Investigative Ophthalmology & Visual Science
|July 1, 1992
PubMed
Summary
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Optisol and Dexsol storage solutions for corneas showed similar deturgescence, but Optisol better prevented swelling during storage. Corneal deturgescence activity decreased over time in both solutions.

Area of Science:

  • Ophthalmology
  • Corneal Preservation
  • Tissue Engineering

Background:

  • Corneal storage media are crucial for maintaining tissue viability and function.
  • Dexsol and Optisol are commonly used storage solutions for donor corneas.
  • Understanding their impact on corneal hydration and deturgescence is vital for transplantation success.

Purpose of the Study:

  • To compare the effects of Dexsol and Optisol on corneal thickness, deturgescence, and passive swelling.
  • To evaluate the long-term corneal deturgescence activity after storage in these media.
  • To determine the superiority of one storage solution over the other in preserving corneal function.

Main Methods:

  • Rabbit and human corneas were stored in Dexsol or Optisol at 4°C for up to 14 days.

Related Experiment Videos

  • Corneal thickness changes were monitored during storage.
  • Net deturgescence and passive swelling were assessed through post-storage culture experiments.
  • Ouabain was used to measure passive swelling characteristics.

    • Main Results:

    • Corneas in Dexsol showed an initial thickness decrease of 10 microns, while Optisol caused a 35-micron decrease.
    • Both solutions demonstrated similar net deturgescence and passive swelling patterns over time.
    • Deturgescence rates decreased with longer storage, correlated with increased passive swelling.
    • Total deturgescence activity after 14 days was 63% for Dexsol and 69% for Optisol compared to controls.
    • Optisol was superior to Dexsol in preventing corneal swelling during storage.

    Conclusions:

    • Optisol offers no significant advantage over Dexsol in retaining post-storage corneal deturgescence activity.
    • Optisol demonstrates superior efficacy in minimizing corneal swelling during storage compared to Dexsol.
    • Storage duration negatively impacts deturgescence, primarily due to increased passive swelling over time.