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Related Experiment Videos

NADPH oxidases in Eukaryotes: red algae provide new hints!

Cécile Hervé1, Thierry Tonon, Jonas Collén

  • 1UMR7139 (CNRS-UPMC-LIA DIAMS), Station Biologique, F-29682, Roscoff Cedex, France.

Current Genetics
|December 14, 2005
PubMed
Summary

Researchers identified a novel NADPH oxidase gene, Ccrboh, in the red alga Chondrus crispus. This gene is upregulated during pathogen attack, suggesting a role in the alga's defense mechanisms.

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Area of Science:

  • Marine Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The red macro-alga Chondrus crispus produces superoxide radicals when exposed to its pathogenic endophyte Acrochaete operculata.
  • Enzymes mediating this response have not been previously identified in red algae.

Purpose of the Study:

  • To isolate and characterize a gene involved in the respiratory burst pathway in Chondrus crispus.
  • To investigate the evolutionary context of NADPH oxidases in algae and eukaryotes.

Main Methods:

  • Isolation of a gene encoding a respiratory burst oxidase homologue (Ccrboh) from C. crispus.
  • Bioinformatic analysis of Ccrboh, including domain organization and phylogenetic relationships.
  • Transcriptional analysis of Ccrboh under various stress conditions (pathogen infection, herbicides, jasmonates, PUFAs).

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Main Results:

  • A single-copy gene, Ccrboh, encoding an 825-amino acid polypeptide was isolated from C. crispus.
  • Homologous NADPH oxidase sequences were identified in other red and brown algae, as well as diatoms.
  • Ccrboh transcription was significantly upregulated during A. operculata infection and upon exposure to atrazine, methyl jasmonate, and PUFA-derived hydroxyperoxides.

Conclusions:

  • The Ccrboh gene represents a key component of the oxidative stress response in Chondrus crispus.
  • The findings provide insights into the deep evolutionary history of NADPH oxidases in eukaryotes.
  • Further research into red algal NADPH oxidases can illuminate plant and animal immune system evolution.