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Related Experiment Videos

Multiple syringe inoculator for agar plates.

T Kaneko1, M Holder-Franklin, M Franklin

  • 1Microbiology Research Laboratory, Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 5A3.

Applied and Environmental Microbiology
|April 1, 1977
PubMed
Summary
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A new manual multiple syringe inoculator allows for 9 or 21 simultaneous inoculations. This device offers rapid, reproducible microbial plating with no needle-agar contact, simplifying laboratory workflows.

Area of Science:

  • Microbiology
  • Laboratory Equipment Design

Background:

  • Microbiological sample inoculation is a fundamental laboratory technique.
  • Current methods can be time-consuming and prone to variability.
  • Standardized inoculation is crucial for reproducible experimental results.

Purpose of the Study:

  • To describe the design and operation of a novel manually operated multiple syringe inoculator.
  • To evaluate the efficiency and reproducibility of the device for microbial plating.
  • To present a simple, cost-effective solution for simultaneous inoculations.

Main Methods:

  • Design and construction of a manually operated multiple syringe inoculator.
  • Testing the device for simultaneous inoculation of 9 or 21 samples.
  • Assessing reproducibility and inoculation time for up to 100 plates.

Related Experiment Videos

  • Verifying that inoculating needles do not contact the agar surface.

    • Main Results:

    • The inoculator can perform 9 or 21 simultaneous inoculations of constant volume.
    • Up to 100 plates can be inoculated within 15 minutes.
    • Excellent reproducibility was achieved in plating.
    • The design ensures no contact between needles and the agar surface.

    Conclusions:

    • The manually operated multiple syringe inoculator is an efficient and reproducible tool for microbial plating.
    • Its simple, inexpensive construction and minimal maintenance requirements make it suitable for various laboratory settings.
    • The device enhances throughput and consistency in microbiological sample preparation.