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Related Experiment Video

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Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
11:43

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Autoclavable dispenser for disposable pipette tips.

S A London1

  • 1Toxic Hazards Division, Aerospace Medical Research Laboratory, Wright-Patterson Air Force Base, Ohio 45433.

Applied and Environmental Microbiology
|April 1, 1977
PubMed
Summary

This study introduces an autoclavable dispenser for disposable pipette tips. It ensures aseptic removal of individual tips, maintaining the sterility of the rest.

Area of Science:

  • Laboratory equipment design
  • Aseptic technique
  • Sterilization methods

Background:

  • Maintaining sterility of laboratory consumables is critical for experimental accuracy.
  • Current methods for handling disposable pipette tips can compromise sterility.
  • A need exists for improved sterile tip dispensing solutions.

Purpose of the Study:

  • To describe a novel autoclavable dispenser for plastic disposable pipette tips.
  • To demonstrate a method for aseptic individual tip removal.
  • To ensure the sterility of remaining pipette tips within the dispenser.

Main Methods:

  • Design and fabrication of an autoclavable pipette tip dispenser.
  • Testing of the dispenser's ability to maintain sterility after autoclaving.

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  • Evaluation of aseptic individual tip removal procedures.

    • Main Results:

    • The developed dispenser is fully autoclavable.
    • Individual pipette tips can be aseptically removed.
    • Sterility of the remaining tips is preserved during dispensing.

    Conclusions:

    • The autoclavable dispenser effectively facilitates aseptic handling of disposable pipette tips.
    • This innovation supports sterile workflows in laboratory settings.
    • The dispenser enhances the reliability of experiments requiring sterile pipette tips.