Related Experiment Video
Updated: Aug 14, 2026

Fast and Accurate Exhaled Breath Ammonia Measurement
Published on: June 11, 2014
Automated enzymatic method for the determination of ammonia: application to rumen fluid, gut fluid, and plasma
A L Baetz1, T D Faidley, M J Allison
1Biochemistry and Physiology Sections, National Animal Disease Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Ames, Iowa 50010.
Abstract:
The enzymatic method for the determination of ammonia with glutamate dehydrogenase has been adapted to the AutoAnalyzer. The automated method was applicable for NH(3) measurement in sheep rumen samples, swine gut fluid samples, and bovine plasma. Results were compared with those of the Conway diffusion method and the manual enzymatic method. The automated method allows 30 samples per h to be analyzed routinely. Values were comparable with those with the manual method, but lower than those with the Conway diffusion method.
More Related Videos
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Related Concept Videos
Inorganic Nitrogen Assimilation
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...