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Hydrolytic enzyme production by Rhizobium
E Martinez-Molina1, V M Morales, D H Hubbell
1Department of Soil Science, University of Florida, Gainesville, Florida 32611.
Applied and Environmental Microbiology
|December 1, 1979
Summary
Rhizobium bacteria, including temperate and tropical strains, possess cellulase and hemicellulase enzymes. These findings suggest novel roles for Rhizobium in plant biomass degradation.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Rhizobium bacteria are known for nitrogen fixation.
- The enzymatic capabilities of Rhizobium beyond nitrogen fixation are not fully understood.
Purpose of the Study:
- To investigate the presence and activity of cellulase and hemicellulase in various Rhizobium strains.
- To explore the potential implications of these enzymes in microbial processes.
Main Methods:
- Utilized cup-plate assays for initial detection of hydrolytic enzyme activity.
- Employed viscometric assays to confirm the presence of cellulase and hemicellulase.
Main Results:
- Cellulase and hemicellulase activity were identified in both temperate (infective and noninfective) and tropical (infective) Rhizobium strains.
- The study confirmed the presence of these key hydrolytic enzymes.
Conclusions:
- Rhizobium strains possess cellulase and hemicellulase, indicating a broader enzymatic repertoire than previously recognized.
- These enzymes may play a role in the degradation of plant cell wall components, with potential ecological and biotechnological implications.