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Updated: Aug 14, 2026

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms
Published on: March 1, 2022
Promotion of infection thread formation by substances from Rhizobium
1Department of Biology, Faculty of Science, Kagoshima University, Kagoshima 890, Japan.
Abstract:
An extrinsic substance (ES-6000) was isolated from the periplasmic space of Rhizobium trifolii (strain 4S) cells by osmotic shock, using a high-density sucrose solution. This substance promoted infection thread formation in root hairs of white clover when inoculated together with the infectious strain (4S). However, ES-6000 obtained from another rhizobial species and from strain A1, which is a noninfectious mutant strain obtained from strain 4S, did not have this effect. The promoter in the ES-6000 from strain 4S is a relatively small molecule since it passed through a hollow-fiber membrane (molecular weight, 6,000). This substance was also recognized as an R(f) 0.1 fraction by paper chromatography. Sucrose was effective in promoting nodulation and root elongation.
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