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Related Experiment Videos

Evidence for cometabolism in sewage.

S N Jacobson1, N L O'mara, M Alexander

  • 1Laboratory of Soil Microbiology, Department of Agronomy, Cornell University, Ithaca, New York 14853.

Applied and Environmental Microbiology
|November 1, 1980
PubMed
Summary

Microbial cometabolism was demonstrated using a novel procedure, showing herbicides converted to metabolic products without cellular incorporation. This highlights natural ecosystem processes for pesticide degradation.

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Area of Science:

  • Environmental microbiology
  • Biogeochemical cycles

Background:

  • Cometabolism is a key process in natural ecosystems for degrading xenobiotic compounds.
  • Understanding cometabolism is crucial for predicting the fate of pollutants in the environment.

Purpose of the Study:

  • To develop and validate a procedure for demonstrating cometabolism in natural ecosystem models.
  • To investigate the cometabolic potential of sewage microflora for four specific herbicides.

Main Methods:

  • Incubation of C-labeled herbicides (trifluralin, profluralin, fluchloralin, nitrofen) with sewage under aerobic and anaerobic conditions for 88 days.
  • Analysis of metabolic product formation and substrate carbon incorporation into cellular constituents.
  • Comparison of conversion rates in nonsterile versus sterile sewage and with concentrated microflora.

Main Results:

  • Formation of metabolic products from all tested herbicides in nonsterile sewage, with yields exceeding 87-90% for most.
  • No incorporation of herbicide carbon into the nucleoside pool of sewage microflora.
  • Enhanced conversion rates with a 40-fold concentration of sewage microflora.

Conclusions:

  • The developed procedure effectively demonstrates cometabolism in natural ecosystem models.
  • Sewage microflora exhibit significant cometabolic activity towards the tested herbicides.
  • Cometabolism, rather than direct assimilation, is the primary pathway for herbicide transformation by these microbes.

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