Related Experiment Video
Updated: Aug 14, 2026

Synthesis of Esters Via a Greener Steglich Esterification in Acetonitrile
Published on: October 30, 2018
Catabolism of substituted benzoic acids by streptomyces species
J B Sutherland1, D L Crawford, A L Pometto
1Department of Bacteriology and Biochemistry, Idaho Agricultural Experiment Station, University of Idaho, Moscow, Idaho 83843.
Abstract:
Four thermotolerant actinomycetes from soil, identified as Streptomyces albulus 321, Streptomyces sioyaensis P5, Streptomyces viridosporus T7A, and Streptomyces sp. V7, were grown at 45 degrees C in media containing either benzoic acid or hydroxyl- and methoxyl-substituted benzoic acids as the principal carbon sources. Benzoic acid was converted to catechol; p-hydroxybenzoic, vanillic, and veratric acids were converted to protocatechuic acid; and m-hydroxybenzoic acid was converted to gentisic acid. Catechol, protocatechuic acid, and gentisic acid were cleaved by catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase, and gentisate 1,2-dioxygenase, respectively. Dioxygenases appeared only in induced cultures. m-Hydroxybenzoic, m-anisic, and p-anisic acids were gratuitous inducers of dioxygenases in some strains. One strain converted vanillic acid to guaiacol.
Related Concept Videos
Electrophilic Aromatic Substitution: Sulfonation of Benzene
Amino Acid Catabolism
Nucleophilic Aromatic Substitution: Elimination–Addition
Production of Antibiotics
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
Electrophilic Aromatic Substitution: Nitration of Benzene
