Detection of rotavirus in sewage

FOR DETECTION OF ROTAVIRUS, DOMESTIC SEWAGE WAS CONCENTRATED BY TWO DIFFERENT METHODS: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.