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Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Bacterial Phylum Spirochaetes

Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased by a...
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The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...

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Specific Lysogenicity in Streptomyces azureus.

S Ogata1, S Yoshino, H Suenaga

  • 1Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Kyushu University, Fukuoka, 812, Japan.

Applied and Environmental Microbiology
|July 1, 1981
PubMed
Summary

Lysogenic Streptomyces azureus harbors temperate phage SAt2. Virulent phage mutants infect aerial mycelia, causing plaques on solid cultures but not in liquid media.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacteriology

Background:

  • Streptomyces azureus produces the antibiotic thiostrepton.
  • Spontaneous plaque formation observed in solid cultures of S. azureus.
  • Plaques disrupt normal mycelial development and sporulation.

Purpose of the Study:

  • Investigate the cause of spontaneous plaque formation in S. azureus cultures.
  • Characterize the phage particles observed in plaques.
  • Determine the interaction between S. azureus and the observed phage.

Main Methods:

  • Serial subculturing of S. azureus on solid media.
  • Electron microscopy of plaque contents.
  • Comparison of phage propagation in liquid and solid cultures.
  • Lysogenic conversion studies.

Main Results:

  • Plaques increased with serial subculturing, affecting aerial mycelia but not substrate mycelia.
  • Phage particles, headless tails, and tail tips were identified in plaques.
  • Phage propagation occurred on solid media but not in liquid media.
  • S. azureus mycelia and spores harbored phage-producing abilities.

Conclusions:

  • S. azureus is lysogenic, harboring temperate phage SAt2.
  • Virulent mutants of SAt2 infect aerial mycelia and sporulating hyphae.
  • Phage propagation is specific to solid culture conditions for this system.