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Attachment and rosette formation by hyphomicrobia
1Faculty of Medicine, Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
Applied and Environmental Microbiology
|November 1, 1981
Summary
Hyphomicrobium sp. strain ZV580 forms rosettes via cell-surface adhesion. Sugars like galactose and mannose, and proteins like concanavalin A, inhibit this process, suggesting a glycoprotein adhesin involved in bacterial surface interactions.
Area of Science:
- Microbiology
- Cell Biology
- Biochemistry
Background:
- Hyphomicrobium sp. strain ZV580 exhibits cell-cell interactions leading to rosette formation.
- Understanding these interactions is crucial for comprehending bacterial adhesion and biofilm development.
Purpose of the Study:
- To investigate the mechanisms of reversible and irreversible sorption of Hyphomicrobium sp. strain ZV580 to surfaces.
- To identify compounds and conditions affecting rosette formation in this bacterial strain.
Main Methods:
- Utilized swarmer cell populations of Hyphomicrobium sp. strain ZV580.
- Examined the effects of various compounds (monosaccharides, lectins, antibiotics, proteases) and physical conditions on cell aggregation.
Main Results:
- Galactose and mannose completely inhibited rosette formation.
- Concanavalin A and chloramphenicol also prevented rosette aggregation.
- Trypsin and pronase treatments dissociated existing rosettes.
Conclusions:
- The adhesin mediating cell-cell interactions is likely a glycoprotein or peptido-polysaccharide.
- Bacterial flagella may play a role in transporting this adhesin to the cell surface.