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Related Experiment Videos

Methods for studying aquatic bacteriophage ecology.

S B Primrose1, N D Seeley, K B Logan

  • 1Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.

Applied and Environmental Microbiology
|March 1, 1982
PubMed
Summary
This summary is machine-generated.

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New methods can identify and count various bacteriophages in water samples. These techniques help measure virus inactivation and assess fecal pollution levels for environmental monitoring.

Area of Science:

  • Environmental microbiology
  • Virology
  • Molecular biology

Background:

  • Bacteriophages are crucial indicators of water quality and environmental health.
  • Accurate enumeration of diverse bacteriophage types is essential for environmental monitoring and risk assessment.
  • Existing methods may lack the specificity or comprehensiveness needed to identify multiple phage groups.

Purpose of the Study:

  • To develop and present novel methods for enumerating diverse bacteriophage populations in environmental water samples.
  • To demonstrate the applicability of these methods for identifying specific phage groups, including F-specific and plasmid-specific phages.
  • To explore the utility of these enumeration techniques for environmental applications like pollution assessment.

Main Methods:

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  • Utilized mixed indicators, equilibrium buoyant-density centrifugation, and enzyme/inhibitor treatments.
  • Employed specific hybridization probes, individually or in combination, for enhanced specificity.
  • Applied these techniques to virus concentrates prepared from large volumes of water (120 liters).

    • Main Results:

    • Successfully enumerated filamentous and isometric male-specific (F-specific) phages.
    • Differentiated and quantified various classes of P-group plasmid-specific phages.
    • Identified and enumerated phiX174-like and lambda-like phages within the samples.

    Conclusions:

    • The developed methods provide a robust framework for comprehensive bacteriophage enumeration in environmental samples.
    • These techniques are adaptable for coliphages and potentially other host-infecting viruses.
    • Applications include effective measurement of virus inactivation and assessment of fecal pollution in aquatic environments.