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Nitrogenase Activity Associated with Halodule wrightii Roots.

G W Smith1, S S Hayasaka

  • 1Department of Microbiology, Clemson University, Clemson, South Carolina 29631.

Applied and Environmental Microbiology
|June 1, 1982
PubMed
Summary
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Nitrogen fixation in seagrass roots was measured using acetylene reduction assays. Highest activity occurred in summer, with Klebsiella pneumoniae identified as a key bacterium.

Area of Science:

  • Marine biology
  • Microbiology
  • Biogeochemistry

Background:

  • Seagrasses are vital marine ecosystems.
  • Nitrogen fixation is crucial for nutrient cycling in marine environments.
  • Understanding nitrogen fixation in seagrasses like Halodule wrightii is important for coastal ecosystem health.

Purpose of the Study:

  • To quantify nitrogen fixation associated with Halodule wrightii roots.
  • To determine the optimal conditions for nitrogenase activity.
  • To identify microorganisms responsible for nitrogen fixation in this seagrass.

Main Methods:

  • Acetylene reduction assays were used to measure nitrogen fixation rates.
  • Experiments were conducted under aerobic and anaerobic conditions.

Related Experiment Videos

  • Temperature effects and seasonal variations were investigated.
  • Bacterial isolates from seagrass roots were identified.

    • Main Results:

    • Acetylene reduction rates were higher in aerobic conditions and linear for up to 5 days.
    • Optimal temperature for acetylene reduction was 35°C, with activity between 15-35°C.
    • Seasonal variation showed peak nitrogenase activity in summer (23 µg N(2)/m²/day).
    • Klebsiella pneumoniae type 4B was isolated from surface-sterilized roots.

    Conclusions:

    • Halodule wrightii roots support significant nitrogen fixation.
    • Environmental factors like temperature and oxygen availability influence nitrogenase activity.
    • Klebsiella pneumoniae is a key diazotroph associated with this seagrass species.
    • These findings contribute to understanding nutrient dynamics in seagrass ecosystems.